Quick answer: there is no approved kisspeptin dosage. In human research, kisspeptin-10 was given mostly by vein, at single doses of 0.01 to 3.0 micrograms per kg in men or as infusions of 1.5 to 4 micrograms per kg per hour, and the strongest single-dose effect came at 1 microgram per kg.[1] Women’s studies mostly used the longer form, kisspeptin-54, at 0.2 to 12.8 nmol per kg under the skin.[8][9] Every dose was given in a research unit, not at home.
This guide lists the doses tested in men and women, what happened at each, and why repeated dosing backfired in several studies. For background, see our kisspeptin-10 profile, our guide to kisspeptin for women, and the sexual health peptides hub.
Kisspeptin-10 vs kisspeptin-54: why the form matters
Kisspeptin is a brain hormone that switches on the reproductive system. It triggers GnRH, which makes the pituitary release LH and FSH; in men, LH then raises testosterone.[1] Two forms appear in research:
- Kisspeptin-10 is the short 10-amino-acid fragment. It is the form FDA reviewed for use in compounding.[17]
- Kisspeptin-54 is the longer form that circulates in blood. It lasted about 28 minutes in blood in one study,[3] which is why researchers chose it for injections under the skin.
Doses are not interchangeable between the two. Most studies dosed by body weight in nanomoles (nmol) or micrograms per kg. When given by vein at matched nmol doses for 3 hours, the two forms raised LH by similar amounts, and natural GnRH raised it 2 to 3 times more than either.[2]
Kisspeptin dosage in men
| Study | Form and route | Dose |
|---|---|---|
| Healthy men, Edinburgh | Kisspeptin-10, IV bolus | 0.01 to 3.0 micrograms/kg; peak effect at 1 microgram/kg[1] |
| Healthy men, Edinburgh | Kisspeptin-10, IV infusion | 1.5 or 4 micrograms/kg per hour, up to 22.5 hours[1] |
| Men with type 2 diabetes and low testosterone | Kisspeptin-10, IV | 0.3 microgram/kg bolus; 4 micrograms/kg per hour for 11 hours[4] |
| Healthy men, London | Kisspeptin-54, IV infusion | 4 pmol/kg per minute for 90 minutes[3] |
| Healthy men, London (2026) | Kisspeptin-10, under the skin by pump | 1.25 to 10 nmol/kg per hour for 8 hours; then 150 nmol per hour, 8 hours a day for 12 days[5] |
| Men with low sexual desire | Kisspeptin-54, IV infusion | 1 nmol/kg per hour for 75 minutes[15] |
What those doses did:
- Single IV doses: at 1 microgram per kg, LH rose from 4.1 to 12.4 IU/L within 30 minutes. The higher 3 micrograms per kg dose produced a smaller rise, so more was not better.[1]
- Infusions: 4 micrograms per kg per hour for 22.5 hours raised testosterone from 16.6 to 24.0 nmol/L.[1] In men with type 2 diabetes, an 11-hour infusion raised it from 8.5 to 11.4 nmol/L.[4]
- Under the skin by pump: daily 8-hour infusions kept LH raised for 12 days. When the pump ran nonstop at 180 nmol per hour for 5 days, testosterone stayed up but LH fell back to placebo levels.[5]
The 12-day pump study is the closest thing to longer-term dosing in men, and it involved only 15 men.[5] For testosterone options with stronger evidence, see our guide to peptides for testosterone and peptides for male fertility.
Kisspeptin dosage in women
| Study | Form and route | Dose |
|---|---|---|
| Healthy women, cycle-phase study | Kisspeptin-54, under the skin | 0.2 to 6.4 nmol/kg once; 0.4 nmol/kg in each cycle phase[8] |
| Healthy women, early cycle | Kisspeptin-10, IV, under the skin or infusion | Up to 10 nmol/kg IV, 32 nmol/kg under the skin, 720 pmol/kg per minute IV[6] |
| Women before menopause, after menopause, or on hormonal birth control | Kisspeptin-10, IV | 0.3 microgram/kg once[7] |
| Hypothalamic amenorrhea | Kisspeptin-54, under the skin | 6.4 nmol/kg twice daily for 2 weeks; also twice weekly for 8 weeks[12][13] |
| Women with low sexual desire | Kisspeptin-54, IV infusion | 1 nmol/kg per hour for 75 minutes[14] |
Timing in the menstrual cycle changed the response more than dose did:
- Kisspeptin-54: a 0.4 nmol per kg injection raised LH by about 0.1 IU/L in the early (follicular) phase, about 2.2 IU/L in the luteal phase, and about 20.6 IU/L just before ovulation.[8]
- Kisspeptin-10: in a London study, even high IV, injected and infused doses did nothing to LH in the early cycle, but 10 nmol per kg by vein worked just before ovulation.[6]
- Hormone background: in Edinburgh, 0.3 microgram per kg of kisspeptin-10 raised LH in the early cycle, the response was largest after menopause, and there was no significant rise in women taking the combined pill.[7]
IVF trigger doses
In IVF research, a single injection of kisspeptin-54 under the skin replaced the usual hCG “trigger shot” that finishes egg maturation:
- First study (53 women): doses of 1.6, 3.2, 6.4 or 12.8 nmol per kg. Eggs matured at every dose, and more mature eggs were collected at higher doses; 23% had a clinical pregnancy.[9]
- Women at high risk of ovarian hyperstimulation syndrome (60 women): doses of 3.2 to 12.8 nmol per kg. Eggs matured in 95% of women, the best pregnancy rates came at 9.6 nmol per kg, and no woman developed moderate or severe OHSS.[10]
- Second dose (62 women): adding a second 9.6 nmol per kg dose 10 hours after the first raised the share of women with a good egg yield from 45% to 71%.[11]
These were small UK studies, and kisspeptin is not an approved IVF drug. Our peptides for fertility guide covers the approved trigger medicines.
Why more often is not better
Kisspeptin can stop working if it is given too often:
- Twice daily: in women whose periods had stopped (hypothalamic amenorrhea), 6.4 nmol per kg twice a day raised LH by about 24 IU/L on day 1 but only about 2.5 IU/L by the 14th injection day.[12]
- Twice weekly: spacing the same dose to twice a week caused only partial loss of response, and hormone levels were still higher than with saline after 8 weeks. No adverse effects were observed.[13]
- Nonstop pump: in men, a continuous 5-day infusion lost its effect on LH, while 8 hours on and 16 hours off kept working for 12 days.[5]
Sexual desire and nasal spray doses
The headline sexual-desire studies used kisspeptin-54 by IV at 1 nmol per kg per hour for 75 minutes, during brain scans. In 32 women with low sexual desire, it changed brain responses to erotic videos and attractive faces and was well tolerated.[14] In 32 men with low desire, erection response to sexual videos rose by up to 56% versus placebo.[15] These were single infusions, not a take-home treatment. Our best peptides for sexual health guide compares the approved options.
A 2025 study tested kisspeptin-54 nasal spray at 12.8 nmol per kg. It raised LH in healthy men, healthy women and women with hypothalamic amenorrhea, and no adverse events were reported.[16] No nasal kisspeptin product is approved.
Why online “kisspeptin dosage” charts are not study doses
If you have seen a daily self-injection “protocol”, note that we found no published human trial testing kisspeptin-10 that way. The only repeated under-the-skin study used a pump in a research setting.[5]
- Not approved: kisspeptin-10 is not FDA-approved for any use.[17]
- Compounding: FDA placed kisspeptin-10 in 503A category 2, substances that may pose significant safety risks, on September 29, 2023. FDA says it may cause immune reactions by some routes and that it has little or no safety information for the proposed routes.[17] It remained in category 2 on FDA’s May 14, 2026 list.[18]
Our peptide calculator converts milligrams into syringe units, but it cannot tell you whether a dose is safe, effective or what a vial really contains. A clinician should decide whether any hormone treatment is appropriate and set the dose.
Questions to ask a clinician
- Are my symptoms (low testosterone, missed periods, infertility or low desire) caused by a problem kisspeptin would even act on?
- Which approved treatments fit my situation, and what are their doses?
- Would a hormone test, such as a kisspeptin or GnRH stimulation test, help with a diagnosis?
- Am I eligible for a kisspeptin research study?
- If I have already used a kisspeptin product, which blood tests should we check?
This is general information, not medical advice.
